Reference
Koshy, Anita A, Fouts, Ashley E, Lodoen, Melissa B, Alkan, Ozan, Blau, Helen M, Boothroyd, John C. Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions. Nat Methods. 2010;7(4):307-9. doi:10.1038/nmeth.1438
Abstract
We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.